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KMID : 0923620180180020016
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Immune Network
2018 Volume.18 No. 2 p.16 ~ p.16
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Extended Culture of Bone Marrow with Granulocyte Macrophage-Colony Stimulating Factor Generates Immunosuppressive Cells
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Na Hye-Young
Sohn Mo-Ah
Ryu Seul-Hye
Choi Wan-Ho
In Hyun-Ju
Shin Hyun-Soo
Park Chae-Gyu
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Abstract
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Bone marrow-derived dendritic cells (BM-DCs) are generated from bone marrow (BM) cells cultured with granulocyte macrophage-colony stimulating factor (GM-CSF) for a week. In this study we investigated the effect of duration on the BM culture with GM-CSF. Within several months, the cells in the BM culture gradually expressed homogeneous levels of CD11c and major histocompatibility complex II on surface, and they became unable to stimulate allogeneic naive T cells in mixed lymphocyte reaction (MLR). In addition, when the BM culture were sustained for 32 wk or longer, the BM cells acquired ability to suppress the proliferation of allogeneic T cells in MLR as well as the response of ovalbumin-specific OT-I transgenic T cells in antigen-dependent manner. We found that, except for programmed death-ligand 1, most cell surface molecules were expressed lower in the BM cells cultured with GM-CSF for the extended duration. These results indicate that BM cells in the extended culture with GM-CSF undergo 2 distinct steps of functional change; first, they lose the immunostimulatory capacity; and next, they gain the immunosuppressive ability.
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KEYWORD
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Bone marrow, Dendritic cells, Granulocyte-macrophage colony-stimulating factor, Lymphocyte culture test, mixed, Immunosuppression
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